Contents

Preface

1. Performing Quantitative Experiments with Biomolecules

2. Affinity Constants

3. Cooperativity in Binding

4. Rate Constants for Binding and Dissociation

5. Kinetics of Regulation and Cooperativity in Binding Reactions

6. Rate Constants for a Two-step Binding Reaction

7. Data Analysis

Appendix 1: Equation Derivations

Appendix 2: Resources and References

Index